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. 2014 Oct 30;289(50):34838–34850. doi: 10.1074/jbc.M114.601849

FIGURE 2.

FIGURE 2.

SUMO conjugation reaction with USP constructs. a, time course SUMO conjugation reaction with IR1 E3 ligase using different point mutants of the N-terminal region construct of USP28 (USP281–159). b, similar reaction as in a but using Nse2 as SUMO E3 ligase. c, time course SUMO conjugation reaction with IR1 E3 ligase using different point mutants of the catalytic domain construct of USPS28 (USP281–757). d, similar reaction as in a but using Nse2 as SUMO E3 ligase. Reactions were run at 37 °C and stopped with SDS-PAGE loading buffer at marked times. USP281–159 K64R/K115R, USP281–159 K64R/K115R/K99R, and USP281–757 K99R/K115R are shown. DM, double mutant; TM, triple mutant.