FIGURE 3.

Deubiquitinating activity of USP28 on Lys⁴⁸ and Lys⁶³ ubiquitin substrates. a, cartoon representation of the different constructs of USP28 used in the analysis. b, end point activities on Lys⁶³-linked ubiquitin chains of three different dilutions of indicated USP28 constructs after a 30-min reaction. c, end point activities on Lys⁴⁸-linked ubiquitin chains of three different dilutions of USP28 constructs after a 30-min reaction. d, end point activities on Lys⁶³-linked ubiquitin chains of four different dilutions of indicated USP28 constructs after a 30-min reaction. e, end point activities on Lys⁴⁸-linked ubiquitin chains of four different dilutions of indicated USP28 constructs after a 30-min reaction. f, end point activities on Lys⁶³ diubiquitin substrate of four different dilutions of indicated USP28 constructs after a 30-min reaction. g, end point activities on Lys⁴⁸ diubiquitin substrate of four different dilutions of the indicated USP28 constructs after a 30-min reaction. Mw, molecular mass markers.