TABLE 1.

Primers used in this work

Primers	Sequences (5′ → 3′)
USP25-BamHI-F	GGATCCATGACCGTGGAGCAGAACGT
USP28-BamHI-F	GGATCCATGACTGCGGAGCTGCAGCA
USP28-NT-Stop-NotI-R	GCGGCCGCTAAACCATCAACTCTCCTCCAGTCA
USP25-Stop-NotI-R	GCGGCCGCTTATCTTCCATCAGCAGGAG
USP28-Stop-NotI-R	GCGGCCGCTTATTTCACTGTCACAGTTG
U28-K64R-forw	CTCACTGATGAGAGAGTTAGGGAGCCCAGTCAAGACACT
U28-K64R-rev	AGTGTCTTGACTGGGCTCCCTAACTCTCTCATCAGTGAG
U28-K115R-forw	AGTCTACTGGAGTCTCCCAGAATTCAAGCTGATGGAAGA
U28-K115R-rev	TCTTCCATCAGCTTGAATTCTGGGAGACTCCAGTAGACT
MUT-U28NT-K99R-F	CCTTACTCATGATAACAGAGATGATCTTCAGGCTG
MUT-U28NT-K99R-R	CAGCCTGAAGATCATCTCTGTTATCATGAGTAAG G
U28CD757 ORI-F	CAAACACAGCCCGTGCCTATGAGAAGAGCGGTGTAG
U28CDΔNT-F	ATAGGATCCGATGGTTGGCCAGTTGGGCTG
U25FL-R	ATAGCGGCCGTTATCTTCCATCAGCAGGAGTTC
U28FL-R	ATAGCGGCCGCTTATTTCACTGTCACAGTTG
U25CD739E-F	GCAGCAGGAGACCCATAATATCTAGAGCAGCCATC
U25CD739E-R	GATGGCTGCTCTAGATATTATGGGTCTCCTGCTGC
U25CD757Y-F	CAAACACAGCCCGTGCCTAAGAGAAGAGCGGTGTAG
U25CD757Y-R	CTACACCGCTCTTCTCTTAGGCACGGGCTGTGTTTG