TABLE 2.
Identification of SUMOylated residues in USP28
Shown are the sites identified by mass spectrometry in SUMOylated fragments of the USP281–159 and USP281–673 proteins. The number of peptide spectrum matches for the non-SUMOylated or SUMOylated fragments and the motif compared with the linear consensus motif are shown for each. Underline residues are hydrophobic and italic residues are acidic.
| Residue | Peptide spectrum matches |
Motif (ΨKXE) | |
|---|---|---|---|
| Nonmodified | SUMOylated | ||
| SUMOylation sites identified in USP281–159 | |||
| Lys64 | 12 | 1 | VKEP |
| Lys85 | 198 | 4 | NKEV |
| Lys99 | 177 | 257 | NKDD |
| Lys115 | 3 | 7 | PKIQ |
| Lys135 | 67 | 23 | TKRS |
| Lys138 | 0 | 1 | RKRK |
| Lys140 | 0 | 1 | RKRC |
| SUMOylation sites identified in USP281–673 | |||
| Lys64 | 47 | 1 | VKEP |
| Lys85 | 82 | 1 | NKEV |
| Lys99 | 82 | 23 | NKDD |
| Lys115 | 101 | 4 | PKIQ |
| Lys210 | 0 | 2 | EKRN |
| Lys305 | 75 | 1 | GKPF |
| Lys385 | 62 | 1 | NKLE |