TABLE 1.
Primers for RT-PCR/qPCR assay
F is forward primer; R is reverse primer; M is Mus musculus; and D is Danio rerio.
| Tal1/scl-F | CTCGGCAGCGGGTTCTTTGG | M-VEGFR2-R | CAAAGCATTGCCCATTCGAT |
| Tal1/scl-R | GCCTGCTTCACCACCTTCTTG | M-Tie2-F | CAGGCTGATTGTTCGGAGATG |
| Foxc1-F | TAAGCCCATGAATCAGCCG | M-Tie2-R | TGCATTCCCCGGTATCTTCA |
| Foxc1-R | GCCGCACAGTCCCATCTCT | M-Nanog-F | AGGGTCTGCTACTGAGATGCTCTG |
| KLF2-F | CACCGACGACGACCTCAACA | M-Nanog-R | CAACCACTGGTTTTTCTGCCACCG |
| KLF2-R | CGCACAGATGGCACTGGAAT | M-Oct4-F | CTGTAGGGAGGGCTTCGGGCACTT |
| Etv2-F | AGTCGGACCGTGCCAGTTT | M-Oct4-R | CTGAGGGCCAGGCAGGAGCACGAG |
| Etv2-R | TCATGCCCGGCTTTCTCTT | M-GPR126-F | TTCCAGGAACAAATGCACCTTC |
| GATA2-F | GCACCTGTTGTGCAAATTGT | M-GPR126-R | GCACGCCATCACATAACTCACG |
| GATA2-R | GCCCCTTTCTTGCTCTTCTT | VEGFR1-F | ATTTGTGATTTTGGCCTTGC |
| Foxo1-F | AAGGATAAGGGTGACAGCAACAG | VEGFR1-R | CAGGCTCATGAACTTGAAAGC |
| Foxo1-R | TTGCTGTGTAGGGACAGATTATGAC | VEGFR2-F | GTGACCAACATGGAGTCGTG |
| Foxo3-F | AAGCCAGCTACCTTCTCTTCCA | VEGFR2-R | CCAGAGATTCCATGCCACT |
| Foxo3-R | GTGGCTAAGTGAGTCCGAAGTGA | VEGFR3-F | CATCCAGCTGTTGCCCAGG |
| Foxc2-F | GCCCAGCAGCAAACTTTCC | VEGFR3-R | GAGCCACTCGACGCTGATGAA |
| Foxc2-R | CCCGAGGGTCGAGTTCTCA | FGFR1-F | GGAGGATCGAGCTCACTCGTGG |
| STAT1-F | CCCAGAATGCCCTGATTAATG | FGFR1-R | CGGAGAAGTAGGTGGTGTCAC |
| STAT1-R | CTGCAGCTGATCCAAGCAAG | FGFR2-F | GTTAACACCACGGACAAAGAGATTGAG |
| STAT2-F | GTGGTTCAGGAAAGGGCAG | FGFR2-R | AACACGGTTAATGTCATAGG |
| STAT2-R | GGAGGGTGTCCGTTTTCAG | FGFR3-F | CGTGGGGCGAGCGGCAGAAGTC |
| STAT3-F | GGCCCCTCGTCATCAAGA | FGFR3-R | TCCGCTCGGGCCGTGTCCAGTAAG |
| STAT3-R | TTTGACCAGCAACCTGACTTTAGT | FGFR4-F | GTGCAGAAGCTCTCCCGCT |
| STAT4-F | CACCTGCCACATTGAGTCAACTA | FGFR4-R | TGAGCTTGACTTGCCGGAA |
| STAT4-R | TAAGACCACGACCAACGTACGA | EGFR-F | GCGTCTCTTGCCGGAATGT |
| STAT5-F | CGAGTGCAGTGGTGAGATCC | EGFR-R | CTTGGCTCACCCTCCAGAAG |
| STAT5-R | TCCTCTGTCACGGACTCTGC | GPR126-F | ACCTTTCTGTTGGGCATGACA |
| STAT6-F | CTGCCCTCTTCAAGAACCTG | GPR126-R | GAAATAGGTGGTAGAGCTGGA |
| STAT6-R | CATGAACGATGACCACCAAG | D-gpr126-F | CCTGACCGTGGAAACTGCACTAA |
| Hey1-F | GCTGGTACCCAGTGCTTTTGAG | D-gpr126-R | GATGATCCTGAAAGAGCCCCGTC |
| Hey1-R | TGCAGGATCTCGGCTTTTTCT | D-flk1-F | ACGACACTGGCATCTACTCATGC |
| Hey2-F | AGATGCTTCAGGCAACAGGG | D-flk1-R | GCCCTTGTCCTCCATTGTCACAT |
| Hey2-R | CAAGAGCGTGTGCGTCAAAG | D-myod1-F | CTCAAGAGATGCACGTCCACCAA |
| β-Actin-F | GTACGCCAACACAGTGCTG | D-myod1-R | GTCGGATTCGCCTTTTTCTGCTG |
| β-Actin-R | CGTCATACTCCTGCTTGCTG | D-β-actin-F | CGAGCAGGAGATGGGAACC |
| M-VEGFR2-F | GCCCTGCCTGTGGTCTCACTAC | D-β-actin-R | CAACGGAAACGCTCATTGC |
| M-CD31-F | TGCAATTTGAATCCGGACAG | ||
| M-CD31-R | TGCAATTTGAATCCGGACAG |