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. 2014 Oct 14;289(50):34886–34899. doi: 10.1074/jbc.M114.583013

FIGURE 1.

FIGURE 1.

Hbp1 and Hbp2 schematic and sequence alignment. A, a schematic representation of the chromosomal SrtB loci in S. aureus and L. monocytogenes encoding sortase SrtB (red) and the SrtB-anchored proteins IsdC and Hbp1 and Hbp2, respectively (light blue). In both organisms, the regulons also encode SrtA-anchored proteins IsdA, IsdB, Lmo2178, and Lmo2179 (dark blue) and ABC transporter IsdDEF and Lmo2182–2184, respectively (gray). A nearby chromosomal locus codes for the Hup ABC transporter (lmo2429–lmo2431), the primary hemin transporter in L. monocytogenes. B, a schematic representation of Hbp1 and Hbp2 with amino acid sequence numbering for each NEAT domain listed above. C, amino acid sequence alignment of NEAT domains from S. aureus, B. anthracis, and L. monocytogenes. Red highlighting indicates the conserved Tyr that coordinates the iron, yellow highlighting indicates the stabilizing Tyr that H-bonds to the coordinating Tyr, and orange indicates the novel Tyr observed for Hbp2N2.