TABLE 1.

Thermodynamic parameters and affinity data for hemin binding

Protein	KD	ΔH	−TΔSa	ΔG	nb
	nm	kcal/mol	kcal/mol	kcal/mol
Hbp1	25 ± 19	−9.6 ± 0.1	−1.3	−11 ± 0.1	1
Hbp2N2	81 ± 35	−3.5 ± 0.1	−6.3	−9.8 ± 0.1	1
Hbp2N3	48 ± 11	−9.1 ± 0.1	−0.9	−10 ± 0.1	1
Hbp2N2(Y289A)	47,667 ± 14,445	−1.8 ± 0.3	−4.2	−6.0 ± 0.3	1
Hbp2N2(Y280A)	NDc	ND	ND	ND	ND

^a Temperature, 298 K.

^b n refers to the molar ratio hemin:protein.

^c ND refers to binding “not detected.”