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. 2014 Oct 16;289(50):34900–34910. doi: 10.1074/jbc.M114.586362

FIGURE 8.

FIGURE 8.

Identification of the peptide generated by thrombin hydrolysis of phosphomanomutase2. The electrospray ionization-MS-MS spectrum of the peak at m/z 993.0, corresponding to the doubly charged ion of 1984.0 Da attributed to peptide 2–21, shows two major fragments that are compatible with the loss of Ala-Ala from the N terminus (doubly charged y-series fragment at 922.53) and with the loss of Leu-Thr-Ala-Pro-Arg from the C terminus (doubly charged b-series fragment at 715.47).