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cAMP activity assays demonstrating the response of Trp479 mutant receptor semistable cells W479A and W479L compared with wild-type RXFP1 stably expressing cells upon stimulation with H2 relaxin (A) or ML290 (B). The data are expressed as percentages of 5 μm forskolin response pooled from at least three independent assays (actual numbers in Table 2) with triplicate determinations within each assay.
