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. 2014 Oct 28;289(50):34938–34952. doi: 10.1074/jbc.M114.600882

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Streptavidin pulldown assay to determine relaxin binding. A, the first panel shows that EL1/EL2-GB1 does not interact with streptavidin resin. In the second panel, EL1/EL2-GB1 is efficiently pulled down by biotinylated H2 relaxin. This interaction was disrupted with the addition of excess competitor of either unbiotinylated H2 relaxin (third panel) or the LDLa module (fourth panel). B and C, control proteins EL1/EL2-GB1cs (B) and GB1 (C) were unable to bind biotinylated H2 relaxin. U, unbound fraction; B, bound fraction to streptavidin beads. The data are from one representative experiment from three independent experiments.