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. 2014 Oct 28;289(50):34938–34952. doi: 10.1074/jbc.M114.600882

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 9. — Streptavidin pulldown assay to determine relaxin binding to EL1/EL2-GB1 F82A and F82Y. In the first panels, both mutants did not interact with streptavidin resin alone. In the second panels, EL1/EL2-GB1 F82Y was able to be pulled down in the presence of biotinylated H2 relaxin similar to WT-EL1/EL2-GB1. EL1/EL2-F82A was unable to bind biotinylated H2 relaxin as seen with the EL1/EL2-GB1cs and tGB1 proteins. U, unbound fraction; B, bound fraction to streptavidin beads. The data are from one representative experiment of three independent experiments.