TABLE 1.
RNA-seq mapping results
avg., average; nt, nucleotides.
| RNA-seq sample ID | Total reads (avg. length) | Reads after trimminga (avg. length) | Uniquely mapped readsb (percentage) | Nonspecifically mapped reads (percentage) | Unmapped reads (percentage) |
|---|---|---|---|---|---|
| Glu_1 | 27,144,132 (100 nt) | 27,142,479 (98.1 nt) | 21,896,756 (80.7%) | 146,706 (0.5%) | 5,099,017 (18.8%) |
| Glu_2 | 23,398,702 (100 nt) | 23,397,193 (98.1 nt) | 19,166,231 (81.9%) | 160,003 (0.7%) | 4,070,959 (17.4%) |
| Man_1 | 18,539,716 (100 nt) | 18,538,613 (97.9 nt) | 14,737,872 (79.5%) | 297,683 (1.6%) | 3,503,058 (18.9%) |
| Man_2 | 19,986,983 (100 nt) | 19,985,820 (98.1 nt) | 16,100,278 (80.6%) | 297,604 (1.5%) | 3,587,938 (18.0%) |
| GluMan_1 | 19,371,626 (100 nt) | 19,370,646 (98.1 nt) | 14,145,889 (73.0%) | 141,438 (0.7%) | 5,083,319 (26.2%) |
| GluMan_2 | 19,134,272 (100 nt) | 19,132,985 (98.3 nt) | 15,344,904 (80.2%) | 131,959 (0.7%) | 3,656,122 (19.1%) |
| GalMan_1 | 21,673,902 (100 nt) | 21,670,903 (98.0 nt) | 17,476,983 (80.6%) | 112,665 (0.5%) | 4,081,255 (18.8%) |
| GalMan_2 | 25,329,774 (100 nt) | 25,328,422 (98.0 nt) | 19,904,504 (78.6%) | 117,245 (0.5%) | 5,306,673 (21.0%) |
a Reads were trimmed using CLC Genomics Workbench version 5.5.1 with a quality score limit of 0.05 and a maximum number of ambiguities of 2.
b Reads were mapped to the C. polysaccharolyticus KMTHCJ genome using CLC Genomics Workbench version 5.5.1 with a minimum length fraction of 0.9, a minimum similarity fraction of 0.8, and a maximum number of hits for a read of 10.