TABLE 5.

Binding parameters for wild-type and mutant CpMnBP1 interaction with oligosaccharides

ND, could not be determined as isotherms did not indicate any significant binding. Data for mutants were collected using mannotetraose as the ligand.

	N	Ka (×106)	ΔG	ΔH	ΤΔS
			kJ/mol	kJ/mol	kJ/mol
Mannobiose	0.94 ± 0.002	3.30 ± 0.184	−37.2	−81.9 ± 0.34	−44.66
Mannotriose	1.08 ± 0.001	6.39 ± 0.275	−38.9	−70.6 ± 0.17	−31.69
Mannotetraose	1.14 ± 0.003	8.77 ± 0.975	−39.8	−83.7 ± 0.47	−43.91
Mannotetraose (65 °C)	0.862 ± 0.008	1.86 ± 0.39	−40.6	−138.9 ± 2.2	−98.33
Mannopentaose	0.861 ± 0.002	9.32 ± 0.882	−39.6	−79.5 ± 0.41	−39.92
W272A	ND	ND	ND	ND	ND
W25A	0.751 ± 0.05	0.028 ± 0.003	−25.3	−78.6 ± 7.58	−53.27
Q191A	0.835 ± 0.003	6.44 ± 0.685	−38.8	−72.3 ± 0.47	−33.43
N307A	0.680 ± 0.015	0.116 ± 0.009	−28.9	−79.5 ± 2.52	−50.65