FIGURE 1.

Membrane expression of TRPC4 deletion mutants. A, diagram of deletion mutants of TRPC4β. The TRPC4 protein has four ankyrin-like repeats and a coiled-coil domain in their N terminus strands followed by six transmembrane domains and a C terminus that contains the SESD1, CIRB, and PDZ binding domain. B, CFP-tagged deletion mutants of TRPC4β (green) were coexpressed with the PH-YFP (red), selectively binding to PI(4,5)P₂, as an indicator of plasma membrane in HEK293 cells. Wild type TRPC4β channel, Δ1–10, Δ11–20, and Δ720–870 deletion mutants were observed in the plasma membrane of the cells. The scale bar represents 10 μm. The line scan shows CFP-tagged mutants and PH-YFP intensity followed by a white dashed line. C, quantification of the membrane expression of the mutants. This graph shows the fluorescence intensity ratio of the membrane expression of deletion mutants to the whole cell expression. Error bars indicate S.E.