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The important region for the formation of the TRPC4 tetrameric structure. A, in the plasma membrane and B, cytosol, the FRET efficiency between YFP-tagged deletion mutants of the TRPC4β channel and CFP-tagged TRPC4β (WT) channel was quantified by 3-cube FRET. FRET between empty-YFP and TRPC4β (WT)-CFP was a control experiment. Error bars indicate S.E. Here, *, p < 0.05 was statistically significant. n.s., not significant.
