TABLE 2.
Bmax and affinity for CFT and dopamine to HA tagged DAT WT and mutants
Experiments performed and data calculated as described in Table I, only here on a DAT background construct with and inserted HA tag in the ECL 2 (see “Experimental Procedures” for details). The data are shown as means (S.E. interval) calculated from pIC50 ± S.E. ND, not detectable.
| DAT construct | CFT |
||
|---|---|---|---|
| Bmax | Kd | N | |
| fmol/105 cells | nm | ||
| HA-WT | 210 ± 74 | 9.8 (7.5; 13) | 3 |
| HA-R85D | ND | ND | 3 |
| HA-D476R | 43 ± 9 | 31 (21; 47) | 4 |
| HA-R85D/D476R | 35 ± 8 | 20 (12; 36) | 4 |