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. 2014 Oct 7;124(25):3799–3807. doi: 10.1182/blood-2014-04-569392

Figure 2.

Figure 2

Kinetics of tether bond formation and dissociation. (A) Frequency of transient adhesive interactions between microspheres coated with recombinant VWF-A1 protein and surface-immobilized mouse platelets (WSRs of 10 s−1 to 300 s−1). (B-E) Representative graphs depicting the distribution of pause times for more than 30 individual transiently tethered VWF-A1–coated microspheres at the indicated wall shear stresses. The dissociation rate constant (koff) is the negative slope of the linear regression through the experimental data. (F) Effect of force on the GPIbα–VWF-A1 tether bond as a function of koff. Tethering frequency and estimation of koff values for VWF-A1–coated microspheres transiently interacting with surface-immobilized platelets were determined by recording digital images with a Nikon ×10 plan or 60× DIC objective (oil immersion) at 30 fps or 235 fps, respectively (Speed Vision Technologies, San Diego, CA). Data represent the mean ± SD. pN, piconewtons.