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. 2014 Nov 20;3(6):1103–1117. doi: 10.1016/j.stemcr.2014.10.006

Figure 2.

Figure 2

Effects of miR-590 on Cell Cycle and DNA Damage Repair-Related Genes

(A) qRT-PCR verification of transcript levels of the cell cycle regulation and homologous recombination damage repair genes in mESCs transfected with pre-miR-590 or control. Data shown are means ± SD of three independent experiments (n = 3). p < 0.05 and ∗∗p < 0.01.

(B) Western blot analysis for the cell cycle regulation and homologous recombination damage repair genes in mESCs transfected with pre-miR-590 or control.

(C) qRT-PCR verification of mRNA levels of the cell cycle regulation and homologous recombination damage repair genes in mESCs transfected with miR-590-3p/5p inhibitor or control. Data shown are means ± SD of three independent experiments (n = 3). p < 0.05 and ∗∗∗p < 0.001.

(D) Western blot analysis of the cell cycle regulation and homologous recombination damage repair genes in mESCs transfected with miR-590-3p/5p inhibitor or control.