Effects of miR-590 on Cell Cycle and DNA Damage Repair-Related Genes
(A) qRT-PCR verification of transcript levels of the cell cycle regulation and homologous recombination damage repair genes in mESCs transfected with pre-miR-590 or control. Data shown are means ± SD of three independent experiments (n = 3). ∗p < 0.05 and ∗∗p < 0.01.
(B) Western blot analysis for the cell cycle regulation and homologous recombination damage repair genes in mESCs transfected with pre-miR-590 or control.
(C) qRT-PCR verification of mRNA levels of the cell cycle regulation and homologous recombination damage repair genes in mESCs transfected with miR-590-3p/5p inhibitor or control. Data shown are means ± SD of three independent experiments (n = 3). ∗p < 0.05 and ∗∗∗p < 0.001.
(D) Western blot analysis of the cell cycle regulation and homologous recombination damage repair genes in mESCs transfected with miR-590-3p/5p inhibitor or control.