Figure 3.

Overexpression of RB in Late-Passage MSCs Increased the Proliferation Rate and In Vitro Osteogenic and Adipogenic Differentiation Potential, and Reduced Senescence Markers

Late-passage MSCs were transfected with control (CTR) or RB overexpression vectors (OE RB).

(A) MSCs without or with RB overexpression were seeded at 4.5 × 10³ /cm² and cultured for 7 days. MTT assay was performed during the indicated period, and data are shown as the relative fold increase.

(B) MSCs without or with RB overexpression were treated with OIM. Upper: representative pictures of alizarin red S staining at 2 weeks. Lower: optical density measurements of extracted alizarin red S staining.

(C) MSCs without or with RB overexpression were treated with AIM. Upper panel: representative pictures of oil red O staining at 2 weeks. Lower panel: optical density measurements of extracted oil red O staining.

(D–F) Western blot analysis (D), quantitative RT-PCR (E), and β-gal staining (F) of MSCs without or with RB overexpression.

The results are expressed as mean ± SD of three independent experiments. Asterisks indicate significant differences (^∗∗p < 0.01). Scale bar, 50 μm. See also Figure S3.