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. 1982 Jun;69(6):1331–1333. doi: 10.1104/pp.69.6.1331

Induction of δ-Aminolevulinic Acid Synthase Activity and Inhibition of Heme Synthesis in Euglena gracilis by N-Methyl Mesoporphyrin IX 1

Samuel I Beale 1,2, Terrence Foley 1
PMCID: PMC426411  PMID: 16662396

Abstract

N-Methyl mesoporphyrin IX, an inhibitor of heme synthesis, increases extractable δ-aminolevulinic acid (ALA) synthase activity when administered to growing cultures of Euglena gracilis Klebs strain Z Pringsheim in micromolar concentrations. Wild-type light-grown green cells and white aplastidic cells exhibited 2.8-fold and 1.8-fold increases, respectively, in ALA synthase activity within five to six hours after incubation with 4 × 10−6 molar N-methyl mesoporphyrin IX. Protoheme levels were decreased and 59Fe incorporation into heme was inhibited by N-methyl mesoporphyrin IX, indicating that, as in animal cells, N-methyl mesoporphyrin IX acts specifically to block iron insertion into protoporphyrin IX. Chlorophyll synthesis in wild-type cells was not affected within the first 6 hours after administration of N-methyl mesoporphyrin IX.

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Selected References

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  1. BURNHAM B. F., PIERCE W. S., WILLIAMS K. R., BOYER M. H., KIRBY C. K. delta-aminolaevulate dehydratase from Rhodopseudomonas spheroides. Biochem J. 1963 Jun;87:462–472. doi: 10.1042/bj0870462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beale S. I., Castelfranco P. A. The Biosynthesis of delta-Aminolevulinic Acid in Higher Plants: I. Accumulation of delta-Aminolevulinic Acid in Greening Plant Tissues. Plant Physiol. 1974 Feb;53(2):291–296. doi: 10.1104/pp.53.2.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Beale S. I., Foley T., Dzelzkalns V. delta-Aminolevulinic acid synthase from Euglena gracilis. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1666–1669. doi: 10.1073/pnas.78.3.1666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Beale S. I. The biosynthesis of delta-aminolevulinic acid in Chlorella. Plant Physiol. 1970 Apr;45(4):504–506. doi: 10.1104/pp.45.4.504. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Castelfranco P. A., Jones O. T. Protoheme turnover and chlorophyll synthesis in greening barley tissue. Plant Physiol. 1975 Mar;55(3):485–490. doi: 10.1104/pp.55.3.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. De Matteis F., Gibbs A. H., Smith A. G. Inhibition of protohaem ferro-lyase by N-substituted porphyrins. Structural requirements for the inhibitory effect. Biochem J. 1980 Sep 1;189(3):645–648. doi: 10.1042/bj1890645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Duggan J., Gassman M. Induction of porphyrin synthesis in etiolated bean leaves by chelators of iron. Plant Physiol. 1974 Feb;53(2):206–215. doi: 10.1104/pp.53.2.206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hatch T., Lascelles J. Accumulation of porphobilinogen and other pyrroles by mutant and wild type Rhodopseudomonas spheroides: regulation by heme. Arch Biochem Biophys. 1972 May;150(1):147–153. doi: 10.1016/0003-9861(72)90021-5. [DOI] [PubMed] [Google Scholar]
  9. Lascelles J., Hatch T. P. Bacteriochlorophyll and heme synthesis in Rhodopseudomonas spheroides: possible role of heme in regulation of the branched biosynthetic pathway. J Bacteriol. 1969 May;98(2):712–720. doi: 10.1128/jb.98.2.712-720.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lilley R. M. Isolation of Functionally Intact Rhodoplasts from Griffithsia monilis (Ceramiaceae, Rhodophyta). Plant Physiol. 1981 Jan;67(1):5–8. doi: 10.1104/pp.67.1.5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MAUZERALL D., GRANICK S. The occurrence and determination of delta-amino-levulinic acid and porphobilinogen in urine. J Biol Chem. 1956 Mar;219(1):435–446. [PubMed] [Google Scholar]
  12. Nadler K., Granick S. Controls on chlorophyll synthesis in barley. Plant Physiol. 1970 Aug;46(2):240–246. doi: 10.1104/pp.46.2.240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Richard F., Nigon V. La syntheèse de l'acide delta-aminolévulinique et de la chlorophylle lors de l'éclairement d'Euglena gracilis étiolées. Biochim Biophys Acta. 1973 Jun 20;313(1):130–149. [PubMed] [Google Scholar]
  14. Stillman L. C., Gassman M. L. Protoheme extraction from plant tissue. Anal Biochem. 1978 Nov;91(1):166–172. doi: 10.1016/0003-2697(78)90827-8. [DOI] [PubMed] [Google Scholar]
  15. URATA G., GRANICK S. Biosynthesis of alpha-aminoketones and the metabolism of aminoacetone. J Biol Chem. 1963 Feb;238:811–820. [PubMed] [Google Scholar]

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