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Effect of homocysteine on the expression of collagen type 1 and AT1R in cultured adventitial fibroblasts. (A) Western blot indicated collagen type 1 and AT1R levels in cells treated with l-Hcy(100 µmol/L) for different periods of time, or pretreated with valsartan (100 nmol/L) for 30 min followed by l-Hcy(100 µmol/L) treatment for 48 h (n = 4).Densitometric analyses of collagen type 1 and AT1R were performed against β-tubulin. * p < 0.05 compared with controls (B,C).
