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. 2014 Oct 31;15(11):19832–19846. doi: 10.3390/ijms151119832

Figure 2.

Figure 2

Effect of Dexamethasone on cyclin-dependent kinase inhibitor 1A (CDKN1A), cyclin-dependent kinase inhibitor 1B (CDKN1B), growth arrest and DNA-damage 45A (GADD45A) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and expression. RTqPCR analysis was performed in control and experimental SUP-T1 cells collected after 24 h from Dex treatment. GAPDH has long been used as a default reference gene in quantitative mRNA profiling experiments; however, since its expression varied in cancer as for many other genes, was preferred absolute quantification and untreated SUP-T1 cells were used for CT comparison [28]. In ordinate, mRNA relative expression = mRNA of treated cells/mRNA of controls. Data are expressed as the mean ± S.D. of 3 independent experiments performed in three PCR replicates.