Figure 7.
Quantitative RT-PCR was used to assess the transcript levels of CaTIP1-1, NtCAT, NtSOD and NtPOD in the leaves of the transgenic lines and the wild-type. The relative fold differences in the mRNA levels were calculated using the 2−∆∆Ct formula with NtL25 as the internal control. Bars with different lower case letters in each group were significantly different, as determined using Duncan’s multiple range test (p < 0.05). S indicates 0.15 M NaCl stress ((A) wild-type and (C) transgenic plants); M means 0.25 M mannitol stress ((B) wild-type and (D) transgenic plants).