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. 2014 Nov 7;15(11):20413–20433. doi: 10.3390/ijms151120413

Figure 1.

Figure 1

Prediction of the signal peptide (SP) on the N-terminus of Malus xiaojinensis iron-regulated transporter 1 (Mx IRT1). (A) Common features of ER (Endoplasmic reticulum)-processed SPs include a hydrophobic center region, surrounded by regions enriched in polar amino acids on both sides (blue: Hydrophobic amino acids; orange: Polar amino acids; red: Positive amino acids; and pink: Negative amino acids); (B) Mx IRT1 SP was predicted using SignalP4.1 [17]. C-score represents the raw cleavage site score (red); S-score indicates the SP score (green); and Y-score represents the combined cleavage site score (blue), which is a combination (geometric average) of the C-score and the slope of the S-score, providing a better cleavage site prediction than the raw C-score alone; and (C) Alignment of the predicted SP region with published IRT1 amino acid sequences, produced using ClustalW2 [19]. Identical residues are shown on a black background, and conservative substitutions are shown on a gray background.