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. 2014 Nov 7;15(11):20500–20517. doi: 10.3390/ijms151120500

Figure 4.

Figure 4

(a) Effect of sorafenib on phosphorylation of ERK, MEK and AKT in T24 and VMCub1 cell lines after 24 h treatment; and (b) Effect of sorafenib on phosphorylation of ERK, MEK and AKT in VMCub1 cells over time. The lanes marked “Co” contain extracts from T24 cells treated with 20 µM sorafenib for 24 h as positive controls. Note that the same extracts were analyzed on two different gels to avoid overlay by residual signals during the analysis of phosphoproteins.