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. 2014 Nov 7;15(11):20500–20517. doi: 10.3390/ijms151120500

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

(a) Effects of sorafenib, vorinostat, and the combination of both compounds on anti-apoptotic factors myeloid cell leukemia 1 (Mcl-1), B-cell lymphoma-X_L (Bcl-X_L), and c-FLIP. α-Tubulin was detected as a loading control. Cells were treated with sorafenib and/or vorinostat for 24 h with the indicated dosages of each compound. α-Tubulin was detected as a loading control; (b) Effects of sorafenib, vorinostat, and the combination of both compounds on cleavage of caspase 3, caspase 8 and poly(ADP)-ribose polymerase (PARP). Arrows indicate the expected positions of cleaved products (the blots are deliberately overexposed to visualize these). α-Tubulin was detected as a loading control. Cells were treated with sorafenib and/or vorinostat for 24 h with the indicated dosages of each compound; and (c) Effects of sorafenib, vorinostat, and the combination of both compounds (positive control) on cleavage of caspase 3, caspase 8 and PARP using additional anti-bodies against cleaved proteins. Bortezomib was used as positive control as described in [43]. Arrows indicate the expected positions of cleaved products. α-Tubulin was detected as a loading control. Cells were treated with bortezomib, sorafenib, and/or vorinostat for 24 h using the indicated concentrations of each compound.

(a) Effects of sorafenib, vorinostat, and the combination of both compounds on anti-apoptotic factors myeloid cell leukemia 1 (Mcl-1), B-cell lymphoma-X_L (Bcl-X_L), and c-FLIP. α-Tubulin was detected as a loading control. Cells were treated with sorafenib and/or vorinostat for 24 h with the indicated dosages of each compound. α-Tubulin was detected as a loading control; (b) Effects of sorafenib, vorinostat, and the combination of both compounds on cleavage of caspase 3, caspase 8 and poly(ADP)-ribose polymerase (PARP). Arrows indicate the expected positions of cleaved products (the blots are deliberately overexposed to visualize these). α-Tubulin was detected as a loading control. Cells were treated with sorafenib and/or vorinostat for 24 h with the indicated dosages of each compound; and (c) Effects of sorafenib, vorinostat, and the combination of both compounds (positive control) on cleavage of caspase 3, caspase 8 and PARP using additional anti-bodies against cleaved proteins. Bortezomib was used as positive control as described in [43]. Arrows indicate the expected positions of cleaved products. α-Tubulin was detected as a loading control. Cells were treated with bortezomib, sorafenib, and/or vorinostat for 24 h using the indicated concentrations of each compound.