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. 2014 Nov 11;15(11):20686–20705. doi: 10.3390/ijms151120686

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Berteroin inhibits phosphorylation of transforming growth factor β activated kinase-1 (TAK1), IκB kinase (IKK)α/β, IκBα, AKT, p38 mitogen activated protein kinase (MAPK), and extracellular regulated kinase (ERK)1/2 in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. The cells were treated with various concentrations of berteroin for 40 min. LPS was added and the incubation was continued for an additional 10 min (to determine IRAK1, TLR4, MyD88, P-TAK1, TAK1, P-IKKα/β, IKKα/β, P-IκBα, P-AKT, and AKT) or for 20 min (to determine IκBα, P-p38, p38, P-ERK1/2, and ERK1/2). Western blotting was conducted using total cell lysates (left panel). Densities were calculated using Image J software (right panel). Each bar represents mean ± SEM (n = 3). * Significantly different from the untreated group (p < 0.05). Means with different letter (a, b or c) are significantly different among the berteroin groups (p < 0.05).

Berteroin inhibits phosphorylation of transforming growth factor β activated kinase-1 (TAK1), IκB kinase (IKK)α/β, IκBα, AKT, p38 mitogen activated protein kinase (MAPK), and extracellular regulated kinase (ERK)1/2 in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells. The cells were treated with various concentrations of berteroin for 40 min. LPS was added and the incubation was continued for an additional 10 min (to determine IRAK1, TLR4, MyD88, P-TAK1, TAK1, P-IKKα/β, IKKα/β, P-IκBα, P-AKT, and AKT) or for 20 min (to determine IκBα, P-p38, p38, P-ERK1/2, and ERK1/2). Western blotting was conducted using total cell lysates (left panel). Densities were calculated using Image J software (right panel). Each bar represents mean ± SEM (n = 3). * Significantly different from the untreated group (p < 0.05). Means with different letter (a, b or c) are significantly different among the berteroin groups (p < 0.05).