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Testosterone production by rat Leydig cells from control (CON, unstressed) and stress rats. Leydig cells were stimulated for 3 h without (basal) or with 100 ng/mL LH (LH) or 20 µM 22R-hydroxycholesterol (22R) or both (LH + 22R). (A) Basal; (B) LH; (C) 22R; and (D) LH + 22R. Mean ± SEM (n = 14–16). *, *** indicate significant differences compared to the control at p < 0.05 and p < 0.001, respectively.
