Figure 4.

The effect of H₂O₂ pretreatment on GA-induced PCD. Wheat aleurone layers were pretreated with distilled water (Con), 100 μM H₂O₂ or 1 mM H₂O₂ for 6 h and then transferred to a medium containing 5 mM CaCl₂ and 50 μM GA or ABA. Digital images of fluorescently-labeled wheat aleurone cells after 48 h of incubation are shown in (A), live cells appear green and dead cells appear orange or red; Scale bar = 200 μm; The viability of cells following different treatments was quantified after 48 h of incubation from at least four aleurone layers (B, left). Wheat HO-1 transcripts were measured after 12 h of treatment (B, right). Data are the means ± SD of at least four independent measurements from different experiments. Bars denoted by the different letters are significantly different at p < 0.05, according to Duncan’s multiple test.