Figure 8.

Production of H₂O₂ in wheat aleurone layers. Wheat aleurone layers were pretreated with distilled water (Con), 50 μM CoCl₂ or 50 μM CoPP, 10 μM DPI, 100 μM ZnPPIX alone or a combination for 6 h and then transferred to a medium containing 5 mM CaCl₂ and 50 μM ABA or GA. (A) After 24 h of incubation, samples were incubated with DAB (1 mg/mL) for 1 h. Arrows in the picture indicate aleurone layers. Scale bar = 50 μm; (B) H₂O₂ content was measured spectrophotometrically. Data are the means ± SD of at least three independent measurements from different experiments. Within each set of experiments, bars denoted by different letters are significantly different at p < 0.05, according to Duncan’s multiple tests.