Table 2.
Steady-state kinetic parameters of human recombinant G6PD proteins.
| G6PD | kcat (s−1) | KmG6P (µM) | KmNADP+ (µM) | kcat/KmG6P (s−1·M−1) | kcat/KmNADP+ (s−1·M−1) |
|---|---|---|---|---|---|
| Wild-type | 233 | 38.5 | 6.2 | 6.0 × 106 | 37.8 × 106 |
| Yucatan (I) | 138 | 39.9 | 6.4 | 3.5 × 106 | 21.7 × 106 |
| Valladolid (II) | 96 | 21.5 | 3.6 | 4.4 × 106 | 26.2 × 106 |
| Mexico City (III) | 182 | 24.9 | 9.1 | 7.3 × 106 | 19.1 × 106 |
| Nashville (I) | 119 | 90.6 | 31.2 | 1.3 × 106 | 3.8 × 106 |
The values for the activity of the G6PD enzymes are averages of three independent experiments; in all cases, the standard errors were ±5%. The numbers in parentheses indicate the class of each variant.