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. 2014 Nov 18;15(11):21314–21330. doi: 10.3390/ijms151121314

Figure 4.

Figure 4

SENP1 knockdown induces apoptosis in podocytes through p53 SUMOylation. (A,B) Immunoprecipitation analysis was performed to detect SUMOylation and deSUMOylation levels of p53 protein in MPC5 cells. SUMOylated p53 was increased in the SENP1 shRNA group after treatment with 5 μg/mL PAN for 24 h. MPC5 cells transfected with SENP1 shRNA plasmid were treated with DMSO only for control or with 2 μM ginkgolic acid. This experiment was performed in triplicate; (C) Representative flow cytometric analysis of apoptotic cells stained with Annexin V-FITC and PI after treatment with 2 μM ginkgolic acid in SENP1 knockdown MPC5 cells. Annexin V-positive cells were defined as apoptotic cells; (D) The apoptosis rates of MPC5 cells in each group. All data are presented as the mean ± SEM of three independent experiments. * p < 0.05 vs. PAN or PAN + sh-NC groups; # p < 0.05 vs. PAN + sh-SENP1 group; (E) Representative images of TUNEL/DAPI staining are shown to illustrate apoptotic podocytes. Bar = 100 μm. All results are presented as the mean ± SEM of three independent experiments; (F) Rates of TUNEL positive MPC5 cells in each group. All data are presented as the mean ± SEM of three independent experiments. * p < 0.05 vs. control group; # p < 0.05 vs. PAN or PAN + sh-NC groups.