Table 3.

Distribution of the SW/3/06 virus in experimentally inoculated mice

Inoculation route	Dose (EID 50 )	Hpi	Viral titer (log 10 EID 50 /0.1 mL, mean ± standard deviation)					MLD 50 (EID 50 )	MID 50 (EID 50 )
			Lung	Brain	Liver	Spleen	Kidney
I.n.	104.0	30-48	6.4 ± 0.7	1.9 ± 0.1	2.7 ± 0.6	4.2 ± 1.0	4.1 ± 0.8	2.9	1.7
I.m.	104.0	48-72	6.2 ± 0.8	1.8 ± 0.1	2.0 ± 0.4	3.6 ± 0.5	3.5 ± 1.5

BALB/c mice (five per group) were inoculated intranasally or intramuscularly with 10^4.0 EID₅₀/50 μl of SW/3/06 virus. To assess mouse LD₅₀ (MLD₅₀), the mice were inoculated with 10-fold serial dilutions of 10^6.0 EID₅₀ of SW/3/06 virus (10^0.0 to 10^7.0) via the intranasal route and observed for 14 days. To assess mouse ID₅₀ (MID₅₀), lung tissues were collected from three inoculated mice per group at 3 dpi. The lower virus detection limit was 10^1.75 EID₅₀/g.

Hpi, hour post infection.