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SHAPE and structural analyses of the SL2 domain of the pal II and PBS pal mutants. (A) The SHAPE reactivity of the wild type and mutant RNAs are presented as color-coded histograms. The blue boxes highlight the SL2 loop sequences and the 11 nt PBS pal. (B) The SHAPE-validated wild type SA035 SL2 domain structure and the nucleotides are color-coded according to SHAPE reactivity. (C) The mutant SA046 SL2 structural motif is shown after being constrained by SHAPE data.
