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. 2014 Dec 12;5:718. doi: 10.3389/fpls.2014.00718

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Copyright © 2014 Yokawa, Fasano, Kagenishi and Baluška.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Light environment affects sensitivity of Arabidopsis roots to salinity. Seedlings of Col-0 were firstly grown on 1/2 MS media for 5 days. Bottom part of agar was removed diagonally and new 1/2 MS media containing NaCl was added. Petri dishes were placed either in light or dark condition for 3 days. Roots growing on agar plates in dark/light conditions with 0, 100, 200, and 300 mM NaCl media in the bottom half of Petri dishes. The method used for imposing the root halotropism is following exactly the protocol as published by Galvan-Ampudia et al. (2013). White arrows indicate the gravity vector. All seedlings were grown in vertical position. Bar indicates 1 cm.