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. 2014 Nov 6;111(12):2275–2286. doi: 10.1038/bjc.2014.529

Figure 3.

Figure 3

Gossypol in combination with TMZ inhibits endothelial cell tube formation in vitro. HUVECs were seeded on BD Matrigel in the presence or absence of gossypol (3, 6 or 12 μM) with or without TMZ (5 μM) and incubated for 20 h. Representative images of (A) control, (B) gossypol (3 μM)-, (C) gossypol (6 μM)-, (D) gossypol (12 μM)-, (E) TMZ (5 μM)-, (F) gossypol (3 μM)/TMZ (5 μM)-, (G) gossypol (6 μM)/TMZ (5 μM)- and (H) gossypol (12 μM)/TMZ (5 μM)-treated HUVECs were taken at 20 h time point. Scale bar=100 μm ( × 200 magnification). (I) Relative tubular length (percentage of control) and (J) relative number of branching points (percentage of control) were quantified. Each treatment condition was replicated in at least three wells and in three independent experiments. Mean±s.e.m. are presented. *P<0.05 compared with control, #P<0.05 compared with TMZ treatment, ##P<0.05 compared with gossypol treatment (one-way ANOVA and Tukey's post-hoc test).