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. 2014 Nov 6;111(12):2275–2286. doi: 10.1038/bjc.2014.529

Figure 6.

Figure 6

Gossypol in combination with TMZ inhibits human GBM tumour-derived cell viability. Human GBM tumour-derived multicellular spheroids were exposed to gossypol (0, 3, 12, 48 μM) with or without TMZ (0, 100, 200, 400) for 96 h. GBM spheroid viability was assessed using the MTT assay. Each absorbance value was normalised to spheroid area at day 0. (A) At least three spheroids were used per each condition. Human GBM tumour-derived neurosphere cultures (BT248-luc2 and BT224-luc2) were treated for 96 h with gossypol and/or TMZ, and cell viability was assessed using the MTT assay. (B and C) Viability as compared with non-treated controls is shown. Error bars represent mean±s.e.m., *P<0.05 compared with the control, #P<0.05 compared with TMZ treatment, ##P<0.05 compared with gossypol treatment, ANOVA and post-hoc Tukey.