Figure 4.

HmsB-dependent expression of hmsCDE. Primer extension (A) and LacZ fusion (B) experiments were done for hmsC as described in Figure 3. The 5′ terminus of RNA transcript (i.e., transcription start) of hmsC was indicated by arrow with nucleotide A, and the minus numbers under arrow indicated nucleotide position upstream of hmsC. For Western Blot (C), whole-cell protein extract from WT or ΔhmsB or ΔhmsD (negative control) was loaded for SDS-PAGE and incubated with anti-HmsD antibodies. Noted that the first gene of hmsCED was subjected to gene expression assays (A,B), while diguanylate cyclase HmsD was chosen for protein biosynthesis analysis (C).