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. 2014 Dec 5;15:129. doi: 10.1186/s12863-014-0129-0

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© Faust et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Influence of microsatellite variants on promoter activity. Changes in relative promoter activity of pGL4.10 XYLT1 promoter cloning construct c.-1031 to c.+1_complete (wildtype) were determined in response to insertion of microsatellite variants by site-directed mutagenesis. Promoter activities are expressed relative to the activity of construct c.-1031 to c.+1_complete, which was defined as 100%. Plasmids were transfected into SW1353 cells and promoter activity was determined in lysates of untreated (white bars) or TGF-β1 induced (black bars) cells by dual luciferase assay. Values are means ± SEM of triplicates from at least two or three independent experiments. ns = not significant; *p < 0.05; **p < 0.01; ***p < 0.001 (Mann-Whitney U-test).