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HPLC gradient method optimized to separate PQ, MPTP and MPP+
| Time; min | Flow ml/min | A; % | B; % | C; % |
|---|---|---|---|---|
| 0.00 | 0.3 | 60 | 25 | 15 |
| 5.00 | 0.3 | 50 | 25 | 25 |
| 6.00 | 0.3 | 30 | 25 | 45 |
| 15.00 | 0.3 | 30 | 25 | 45 |
| 16.00 | 0.3 | 60 | 25 | 15 |
| 20.00 | 0.3 | 60 | 25 | 15 |
Eluent A- 0.1 % formic acid in water; Eluent B- 0.1 % formic acid in methanol; Eluent C- 0.1 % formic acid in acetonitrile
