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. Author manuscript; available in PMC: 2014 Dec 12.
Published in final edited form as: Nat Commun. 2014 May 20;5:3839. doi: 10.1038/ncomms4839

Figure 5. Treatment with Chordin-like 1 induces proximal phentoypes in the BP.

Figure 5

(a) Maximal z-projections of the surface of BP explants labeled with phalloidin after 6 DIV in control media or media containing 0.4 μ/mL of Chrdl1. Note the decreased density of hair cells in the distal region in response to treatment with Chrdl1. (b) Hair cell density measured in a 2500 μm2 region at the proximal (black bars) and distal (grey bars) end of the chick BP following 6 DIV in control media, or Chrdl1-media. A significant decrease in hair cell density is observed in the distal region. (c) Lumenal surface areas for hair cells at the proximal (black bars) and distal (grey bars) ends of BP explants following 6 DIV in control media of media containing Chrdl1. Chrdl1 caused a significant increase in lumenal surface area in distal hair cells. Data are presented as mean ± sem. *; p < 0.05 based on Student's t-test. Scale bar is 50 μm. For controls n = 8, and for Chrdl1-treated cultures n = 5. Scale bar in a = 100 μm.