Analysis of CSC marker expression by melanoma cells from cell lines versus FNA biopsy samples. (A) Comparison of percentage of surface marker staining of melanoma cell lines (“lines”, passaged >20 times), fresh melanoma tumor biopsy samples (“FNAs”), and “Early passage”, consisting of cell lines in passage numbers 1,6 and 7.p-Values are shown above each graph and reflect the comparison of the cell lines and the FNAs. (B) Comparison of percentage of Oct3/4 staining (left) and CD90 staining (right). n = 7 cell line samples, 8 FNA samples, and 3 Early passage samples for Oct3/4 and n = 7 cell line samples, n = 5 FNA samples, and n=3 Early passage samples for CD90. (C) ALDH staining of melanoma cell lines (n = 7) and FNA samples (n = 5). *p-Values less than 0.05 were considered significant.