Table 3. Non-synonymous mutations in the S region of Palestinian D3 subgenotype.

nt position	aa position	Reported function/detected in	Reference
528: C/T	T125M	Increases HBsAg reactivity in immunological diagnostic assays	[66]
753: A/T	Y200F	Unknown/antiviral therapy	[64]
762: C/A	P203Q	Causes discrepant results in some HBsAg detection assays	[67]
766: T/A	S204R	Unknown, genotype E	[34]
770:T/A, 771: A/C	Y206T	Unknown	Novel
774: G/A	S207N	Unknown/solely anti-HBc-positive sera, genotype D	[34]

Positions of the nucleotide (nt) mutation and the correlating amino acid (aa) are presented based on their location in the archived GenBank reference JF754625. Corresponding references and proposed functions are provided in the last two columns. Unreported mutations were considered novel.