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. 2014 Dec 12;9(12):e113237. doi: 10.1371/journal.pone.0113237

Figure 1. LRP1 knockdown decreases the expression levels of GluA1 in neurons.

Figure 1

Primary cortical neurons cultured from C57Bl/6 mice were infected with lentivirus carrying LRP1-shRNA or control NT-shRNA on day 8 in vitro (DIV) and then harvested after 2 or 4 days of infection. The expression level of LRP1 in neurons was detected by Western blot (A), and densitometrically quantified (B). (C) The cell viability of neurons was assessed by MTT assay at 2 or 4 days following infection. In LRP1-knockdown neurons, the expression levels of PSD95 (D, E), GluA1 (D, F), and GluA2/3 (D, G) at 4 days post-infection were detected by Western blot and densitometrically quantified. In addition, the mRNA levels of PSD95 (H) and GluA1 (I) were also analyzed by quantitative real-time PCR. The data are plotted as mean ± SD (n = 3). N.S., Not significant; **, p<0.01.