Skip to main content
. 2014 Dec 12;9(12):e113237. doi: 10.1371/journal.pone.0113237

Figure 2. LRP1 interacts with GluA1 and regulates its turnover in neurons.

Figure 2

(A) Brain lysates from wild-type mice were immune-precipitated using specific antibodies against LRP1, GluA1, GluA2/3 or PSD95, and their interactions were examined by Western blot (B–E). After infection with control NT-shRNA or LRP1-shRNA, control and LRP1-knockdown neurons were treated with cycloheximide (CHX), and the levels of GluA1 (C), GluA2/3 (D) and PSD95 (E) were analyzed by Western blot at different time points. (F) LRP1-knockdown neurons were treated with DMSO (control), proteasomal inhibitor lactacystin (Lac; 10 µM) or lysosomal inhibitor bafilomycin A1 (BA1; 5 nM) in addition to CHX. (G) GluA1 and PSD95 levels were analyzed by Western blot, and densitometrically quantified. The data are plotted as mean ± SD (n = 3). *, p<0.05; **, p<0.01.