Figure 2. Altered insulin secretion by MIN6 cells under hypoxia.

(A) Cellular insulin content was examined after MIN6 cells were incubated in normoxia (20% O₂, gray bars) or hypoxia (5% O₂, black bars) for 30 h. Insulin content was standardized by cell number. (B) Glucose-stimulated insulin secretion was examined when MIN6 cells that had been cultured at 20% O₂ or 5% O₂ for 40 h were stimulated with low glucose (2.2 mM glucose, gray bars) or high glucose (22 mM glucose, black bars) for 1 h (n = 4). Secreted insulin was normalized to cellular protein levels. Data are shown as the means ± S.E. (error bars) of values from each group. **, p<0.01; ***, p<0.001. N.S., not significant.