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. 2014 Dec 12;9(12):e114795. doi: 10.1371/journal.pone.0114795

Figure 7. Transgene expression in transduced, sorted, EGFP+ and EGFP− K562 cells.

Figure 7

K562 cells were subjected to one round of transduction with the lentiviruses indicated in the figure. After 5 days the cells were sorted by FACSAriaIII based on EGFP expression (A), and the sorted EGFP-positive (gates 1, 3, 5, 7) and -negative (gates 2, 4, 6, 8) subpopulations were analyzed by western blotting for expression of 3xFLAG-ZNF521 and of EGFP (B). The purity of the sorted populations was subsequently evaluated by flow cytometry and is shown in S3 Figure.