Skip to main content
. 2014 Dec 12;9(12):e114887. doi: 10.1371/journal.pone.0114887

Figure 7. Functional characterization of an antibody with improved binding of cMet.

Figure 7

(A,B) FACS analysis of (A) yeast cells displaying parental antibody, (B) cells displaying the selected antibody labeled with 100 nM cMet-PE and anti-Fc AlexaFluor647. (C) Binding kinetics of Expi293F expressed parental antibody and (D) affinity-matured antibody variant to cMet (100 nM, 50 nM, 10 nM) determined by biolayer interferometry. Association rate constants (kon), dissociation rate constants (kdis) and binding affinities (KD) of both antibodies were determined assuming a 1∶1 binding model.