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. 2014 Dec 12;9(12):e114898. doi: 10.1371/journal.pone.0114898

Table 2. Plasmids: Description of plasmids used in this work. Database Number, plasmid backbone used to clone the indicated genes is specified. Original References for previously used plasmids are indicated. When required, plasmids used during the recombination reaction are also indicated.

Database Nr. Plasmid Backbone Genes cloned Refs. Plasmid used in the recombination reaction
K1127 pUCDM - [17]
K1130 pFL - [17]
K1212 pFL-FLAG - [19]
K1502 pSPL-3xHA - [19]
K1670 pFL-FLAG UTP15 This work.
K1671 pFL-FLAG UTP15, UTP9 This work.
K1672 pUCDM UTP4-TAP This work.
K1673 pUCDM UTP5 This work.
K1682 pUCDM UTP5, UTP4-TAP This work.
K1684 pSPL-3xHA NAN1, UTP10 This work.
K1685 pFL- UTP5, UTP9, UTP15-FLAG This work. Amplification module.
K1721 pFL- UTP4-TAP, UTP5 This work
K1978 pFL-FLAG UTP12, UTP13 This work.
K1979 pUCDM UTP18 This work.
K1980 pUCDM UTP18, PWP2-TAP This work.
K1981 pUCDM UTP18, PWP2 This work.
K1982 pFL- UTP6-HA This work
K1983 pSPL-3xHA UTP6-HA, UTP21 This work.
K1986 pFL- UTP6-HA, UTP21-TAP This work. K1130+K2122
K1987 pFL- UTP6-HA, UTP18, UTP21, PWP2-TAP This work. K1980+K1983
K1991 pFL- UTP6-HA, UTP12-FLAG, UTP13, UTP18, UTP21, PWP2-TAP This work. K1978+K1980+K1983
K1992 pFL- UTP6-HA, UTP12-FLAG, UTP13, UTP18, UTP21, PWP2 This work. K1978+K1981+K1983
K1997 pUCDM UTP5, UTP4 This work.
K1999 pFL-FLAG NAN1, UTP10 This work.
K2000 pFL- UTP4, UTP5, UTP8, UTP9, UTP10, UTP15-FLAG, NAN1-HA This work. K1684+K1685+K1997
K2122 pSPL-3xHA UTP6, UTP21-TAP This work.
K2123 pFL- UTP4-TAP, UTP5, UTP8, UTP9, UTP15-FLAG This work. K1682+K1685
K2124 pFL- UTP5, UTP15-FLAG This work. K1670+K1673
K2126 pFL- UTP5, UTP8, UTP9, UTP15-FLAG This work. K1673+K1685
K2134 pFL- UTP6-HA, UTP18, PWP2-TAP K1130+K1980+K1982
K2135 pFL- UTP6-HA, UTP21-TAP This work. K1130+K2122
K2136 pFL- UTP6-HA, UTP18, UTP21-TAP This work. K1986+K1979
K2137 pFL- UTP6-HA, UTP18, UTP21-TAP, PWP2 This work. K1130+K1980+K1983
K2138 pFL- UTP18 This work. K1130+K1979
K2139 pFL- UTP6-HA, UTP18 This work. K2138+K1982
K2204 pFL-FLAG UTP8, UTP9, This work.
K2209 pFL- UTP4-TAP, UTP5, UTP15-FLAG This work. K1670+K1682
K2212 pFL- UTP4-TAP, UTP8-FLAG, UTP9 This work. K1672+K2204