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. 2014 Dec 8;144(1):116–126. doi: 10.1111/imm.12356

Figure 1.

Figure 1

Titres of antibodies against proteins after conjugation of various haptens. (a) Molecular structures of the tested haptens: (i) mannosamine–biotin adduct (MBA), (ii) mannosamine–desthiobiotin adduct (MDTBA), (iii) mannosamine–lipoic acid adduct (MLA), (iv) glucosamine–biotin adduct (GBA), (v) mannose, (vi) biotin. (b) Mice were immunized twice intramuscularly, at 2-week intervals, with horse IgG (hsIgG) or haptenated hsIgG. Anti-carrier (hsIgG) or anti-hapten antibody titre was determined as the reciprocal value of the twofold serial dilution end-point (twice the negative control OD value) of the serum as tested by ELISA (n = 4 to 6). Bar values represent mean antibody levels (±SD). *P < 0.05 for the differences between antibody titre to hsIgG–MDTBA, hsIgG–MLA and hsIgG–MBA, and to hsIgG, hsIgG–biotin, hsIgG–mannose and hsIgG–GBA.